#2863 SEX-, AGING AND DIABETES-RELATED ALTERATIONS IN GLOMERULAR DIMENSIONS AND PODOCYTE DENSITIES USING DEEP-LEARNING QUANTIFICATION

Abstract Background and Aims Kidney function, as well its morphology, changes markedly with age disorders such diabetes. This process is associated structural functional alterations in cortical juxtamedullary glomeruli. Currently, data on differences glomeruli sex, age, genetic factors, diabetes are limited. In this study, we investigated the abundance morphometry of podocytes mice different ages sex’, suffering from or not using a deep-learning based analysis immuno-stained kidney sections. Methods Male female non-diabetic C57BL/6J type II (db/db) were sacrificed at time points: 4, 10, 20, 30, 34, 40 weeks 6, 24 weeks, respectively. Subsequently, kidneys extracted, embedded paraffin, cut into sections, stained, imaged for histological analysis. We used immunhistochemistry staining Wilms Tumor 1 (WT1) antibody to specifically stain podocyte nuclei. Both manual image segmentation performed analyze total, 4134 glomerular structures detected, was extracted analyzed by an automated algorithm. Results Our study aimed investigate aging- diabetes-related respect loss. Using customized deep learning algorithm, nuclei could be quantified comparable quality time-tedious analysis, which takes approximately minute per glomerulus. Extracted morphometric features showed that had larger cross-sectional area than (Figure 1a). For both slightly increased average aging 1b). As expected, endowment lower density being higher 2a, 2b). Over life-time number glomerulus decreased 2b, 3b). Interestingly, smaller compared males 1c, 3c). However, numbers did differ between male 2c). Finally, found old db/db presented hypertrophy contrast same 1d). Db/db lost 6 density. Surprisingly, loss occurred extent 2d, 3d). Conclusion During early diabetic disease occur. Similar sex’. Hyperfiltration might explain pronounced should represent increase filtration surface handle hyperfiltration. Less relate mouse model still moderate stress during stage disease.